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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó; INIA Treinta y Tres. |
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Fecha : |
07/06/2019 |
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Actualizado : |
18/11/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
IRISARRI, P.; CARDOZO, G.; TARTAGLIA, C.; REYNO, R.; GUTIÉRREZ, P.; LATTANZI, F.; REBUFFO, M.; MONZA, J. |
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Afiliación : |
PILAR IRISARRI, Laboratorio de Microbiología, Departamento de Biología Vegetal, Facultad de Agronomía, UDELAR, URUGUAY; GERONIMO AGUSTIN CARDOZO CABANELAS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CAROLINA TARTAGLIA, Laboratorio de Bioquimica, Departamento de Biología Vegetal, Facultad de Agronomía, UDELAR, Uruguay.; RAFAEL ALEJANDRO REYNO PODESTA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PAMELA GUTIÉRREZ, Laboratorio de Bioquímica, Departamento de Biología Vegetal, Facultad de Agronomía, UDELAR, URUGUAY.; FERNANDO A. LATTANZI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MONICA IRENE REBUFFO GFELLER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JORGE MONZA, Laboratorio de Bioquímica, Departamento de Biología Vegetal, Facultad de Agronomía, UDELAR, URUGUAY. |
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Título : |
Selection of competitive and efficient rhizobia strains for white clover. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Frontiers in Microbiology, 23 April 2019, v. 10, art. 768. DOI: https://doi.org/10.3389/fmicb.2019.00768 |
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Páginas : |
11 p. |
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DOI : |
10.3389/fmicb.2019.00768 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 28 September 2018 // Accepted 26 March 2019 // Published: 23 April 2019. Open Access Journal. Acknowledgments: We are grateful to Verónica Berriel for the N abundance determination and to Carlos Rossi for providing white clover seeds. |
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Contenido : |
The practice of inoculating forage legumes with rhizobia strains is widespread. It is assumed that the inoculated strain determines the performance of the symbiosis and nitrogen fixation rates. However, native-naturalized strains can be competitive, and actual nodule occupancy is often scarcely investigated. In consequence, failures in establishment, and low productivity attributed to poor performance of the inoculant may merely reflect the absence of the inoculated strain in the nodules. This study lays out a strategy followed for selecting a Rhizobium leguminosarum sv. trifolii strain for white clover (Trifolium repens) with competitive nodule occupancy. First, the competitiveness of native-naturalized rhizobia strains selected for their efficiency to fix N2 in clover and tagged with gusA was evaluated in controlled conditions with different soils. Second, three of these experimental strains with superior nodule occupancy plus the currently recommended commercial inoculant, an introduced strain, were tested in the field in 2 years and at two sites. Plant establishment, herbage productivity, fixation of atmospheric N2 (15N natural abundance), and nodule occupancy (ERIC-PCR genomic fingerprinting) were measured. In both years and sites, nodule occupancy of the nativenaturalized experimental strains was either higher or similar to that of the commercial inoculant in both primary and secondary roots. The difference was even greater in stolon roots nodules, where nodule occupancy of the native-naturalized experimental strains was at least five times greater. The amount of N fixed per unit plant mass was consistently higher with native-naturalized experimental strains, although the proportion of N derived from atmospheric fixation was similar for all strains. Plant establishment and herbage production, as well as clover contribution in oversown native grasslands, were either similar or higher in white clover inoculated with the native-naturalized experimental strains. These results support the use of our implemented strategy for developing a competitive inoculant from native-naturalized strains. MenosThe practice of inoculating forage legumes with rhizobia strains is widespread. It is assumed that the inoculated strain determines the performance of the symbiosis and nitrogen fixation rates. However, native-naturalized strains can be competitive, and actual nodule occupancy is often scarcely investigated. In consequence, failures in establishment, and low productivity attributed to poor performance of the inoculant may merely reflect the absence of the inoculated strain in the nodules. This study lays out a strategy followed for selecting a Rhizobium leguminosarum sv. trifolii strain for white clover (Trifolium repens) with competitive nodule occupancy. First, the competitiveness of native-naturalized rhizobia strains selected for their efficiency to fix N2 in clover and tagged with gusA was evaluated in controlled conditions with different soils. Second, three of these experimental strains with superior nodule occupancy plus the currently recommended commercial inoculant, an introduced strain, were tested in the field in 2 years and at two sites. Plant establishment, herbage productivity, fixation of atmospheric N2 (15N natural abundance), and nodule occupancy (ERIC-PCR genomic fingerprinting) were measured. In both years and sites, nodule occupancy of the nativenaturalized experimental strains was either higher or similar to that of the commercial inoculant in both primary and secondary roots. The difference was even greater in stolon roots nodules, where nodule occupan... Presentar Todo |
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Palabras claves : |
BIOLOGICAL NITROGEN FIXATION; INOCULANT; NATIVE-NATURALIZED RHIZOBIA STRAINS; NODULE OCCUPANCY; R. LEGUMINOSARUM SV. TRIFOLII; WHITE CLOVER. |
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Thesagro : |
FIJACION BIOLOGICA DEL NITRÓGENO; NODULACION; TREBOL BLANCO; TRIFOLIUM REPENS. |
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Asunto categoría : |
F60 Fisiología y bioquímica de la planta |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/12797/1/Cardozo-Frontier-Micro-2019.pdf
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Marc : |
LEADER 03468naa a2200361 a 4500
001 1059873
005 2021-11-18
008 2019 bl uuuu u00u1 u #d
024 7 $a10.3389/fmicb.2019.00768$2DOI
100 1 $aIRISARRI, P.
245 $aSelection of competitive and efficient rhizobia strains for white clover.$h[electronic resource]
260 $c2019
300 $a11 p.
500 $aArticle history: Received 28 September 2018 // Accepted 26 March 2019 // Published: 23 April 2019. Open Access Journal. Acknowledgments: We are grateful to Verónica Berriel for the N abundance determination and to Carlos Rossi for providing white clover seeds.
520 $aThe practice of inoculating forage legumes with rhizobia strains is widespread. It is assumed that the inoculated strain determines the performance of the symbiosis and nitrogen fixation rates. However, native-naturalized strains can be competitive, and actual nodule occupancy is often scarcely investigated. In consequence, failures in establishment, and low productivity attributed to poor performance of the inoculant may merely reflect the absence of the inoculated strain in the nodules. This study lays out a strategy followed for selecting a Rhizobium leguminosarum sv. trifolii strain for white clover (Trifolium repens) with competitive nodule occupancy. First, the competitiveness of native-naturalized rhizobia strains selected for their efficiency to fix N2 in clover and tagged with gusA was evaluated in controlled conditions with different soils. Second, three of these experimental strains with superior nodule occupancy plus the currently recommended commercial inoculant, an introduced strain, were tested in the field in 2 years and at two sites. Plant establishment, herbage productivity, fixation of atmospheric N2 (15N natural abundance), and nodule occupancy (ERIC-PCR genomic fingerprinting) were measured. In both years and sites, nodule occupancy of the nativenaturalized experimental strains was either higher or similar to that of the commercial inoculant in both primary and secondary roots. The difference was even greater in stolon roots nodules, where nodule occupancy of the native-naturalized experimental strains was at least five times greater. The amount of N fixed per unit plant mass was consistently higher with native-naturalized experimental strains, although the proportion of N derived from atmospheric fixation was similar for all strains. Plant establishment and herbage production, as well as clover contribution in oversown native grasslands, were either similar or higher in white clover inoculated with the native-naturalized experimental strains. These results support the use of our implemented strategy for developing a competitive inoculant from native-naturalized strains.
650 $aFIJACION BIOLOGICA DEL NITRÓGENO
650 $aNODULACION
650 $aTREBOL BLANCO
650 $aTRIFOLIUM REPENS
653 $aBIOLOGICAL NITROGEN FIXATION
653 $aINOCULANT
653 $aNATIVE-NATURALIZED RHIZOBIA STRAINS
653 $aNODULE OCCUPANCY
653 $aR. LEGUMINOSARUM SV. TRIFOLII
653 $aWHITE CLOVER
700 1 $aCARDOZO, G.
700 1 $aTARTAGLIA, C.
700 1 $aREYNO, R.
700 1 $aGUTIÉRREZ, P.
700 1 $aLATTANZI, F.
700 1 $aREBUFFO, M.
700 1 $aMONZA, J.
773 $tFrontiers in Microbiology, 23 April 2019$gv. 10, art. 768. DOI: https://doi.org/10.3389/fmicb.2019.00768
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Registro original : |
INIA Tacuarembó (TBO) |
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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
30/06/2023 |
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Actualizado : |
30/06/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
REGUEIRO, M.; JORGE-SMEDING, E.; BALDI, F.; IDIARTE BORDA, A.; LÓPEZ-MAZZ, C.; BANCHERO, G. |
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Afiliación : |
MARIEL REGUEIRO, Faculty of Agronomy, UDELAR, Av. Garzón 780, 12900, Uruguay; EZEQUIEL JORGE-SMEDING, Faculty of Agronomy, UDELAR, Av. Garzón 780, 12900, Uruguay; FERNANDO BALDI, School of Agricultural Sciences and Veterinary, Sao Pablo 11884100, Brazil; AGUSTINA IDIARTE BORDA, Faculty of Agronomy, UDELAR, Av. Garzón 780, 12900, Uruguay; CARLOS LÓPEZ-MAZZ, Faculty of Agronomy, UDELAR, Av. Garzón 780, 12900, Uruguay; GEORGGET ELIZABETH BANCHERO HUNZIKER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Programmed parturition assistance (PPA) in primiparous wool-type ewes improves mother-lamb behaviour at lambing. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Applied Animal Behaviour Science. August 2023, Vol. 265, 105980. https://doi.org/10.1016/j.applanim.2023.105980 |
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ISSN : |
0168-1591 (print); 1872-9045 (electronic). |
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DOI : |
10.1016/j.applanim.2023.105980 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 6 January 2023; Received in revised form 6 May 2023; Accepted 4 June 2023; Available online 7 June 2023. -- Corresponding author: E-mail address: mariel@fagro.edu.uy (M. Regueiro). -- |
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Contenido : |
The effect of programmed assistance at parturition on the behaviour of the mother and its offspring in primiparous ewes under extensive rearing was evaluated. Programmed parturition assistance was defined as the manoeuvres performed immediately after the beginning of the expulsion phase to shorten it. Twenty-eight 2-year-old Corriedale primiparous ewes (body condition score (BCS): 3.4 ± 0.1; body weight (BW): 35.5 ± 0.7 kg) with a single foetus were used. Before lambing and considering BW, BCS and sire, the ewes were randomly assigned to (i) Programmed-parturition-assisted (PPA; n = 14): ewes whose lambing was programmed to be assisted or (ii) Not-assisted (NA; n = 14): ewes that were not assisted and their lambs were born through natural labour. The duration of the foetus expulsion phase, maternal behaviour score (MBS, 1-5), onset of grooming, lamb/ewe BW ratio and lamb desertion were determined in the ewes. Birth weight, Apgar test (score 0-10), O2 saturation, meconium-stained coat, latency to first bleat, success to stand and suck, were registered in the lambs. PPA ewes registered shorter duration of labour (19.2 ± 4.2 vs. 42.6 ± 7.8 min), earlier onset of grooming (1.2 ± 0.4 vs. 3.0 ± 0.6 min), higher MBS (4.5 ± 0.1 vs. 3.1 ± 0.4) and did not desert any lamb during the first 72 h from birth. The lambs born to PPA mothers registered higher O2 saturation (97.6 ± 1.0 % vs. 93.4 ± 1.3 %), bleated earlier (2.4 ± 0.5 vs. 4.6 ± 0.8 min), stood up earlier (24.1 ± 4.2 vs. 36.8 ± 8.0 min) and recorded shorter time to suck (36.5 ± 6.7 vs. 71.0 ± 12.9 min). No effect of treatment on Apgar test or meconium-stained coat was observed, but regardless of treatment, meconium-stained lambs had a higher lamb/ewe BW ratio than unstained ones. The reduction of the duration of foetal expulsion phase, through programmed parturition assistance, positively affected the vigour of the lambs as well as the maternal behaviour of primiparous ewes, which in turn would increase the chances of lamb survival and ultimately, improve the welfare of the ewe-lamb unit. © 2023 Elsevier B.V. All rights reserved. MenosThe effect of programmed assistance at parturition on the behaviour of the mother and its offspring in primiparous ewes under extensive rearing was evaluated. Programmed parturition assistance was defined as the manoeuvres performed immediately after the beginning of the expulsion phase to shorten it. Twenty-eight 2-year-old Corriedale primiparous ewes (body condition score (BCS): 3.4 ± 0.1; body weight (BW): 35.5 ± 0.7 kg) with a single foetus were used. Before lambing and considering BW, BCS and sire, the ewes were randomly assigned to (i) Programmed-parturition-assisted (PPA; n = 14): ewes whose lambing was programmed to be assisted or (ii) Not-assisted (NA; n = 14): ewes that were not assisted and their lambs were born through natural labour. The duration of the foetus expulsion phase, maternal behaviour score (MBS, 1-5), onset of grooming, lamb/ewe BW ratio and lamb desertion were determined in the ewes. Birth weight, Apgar test (score 0-10), O2 saturation, meconium-stained coat, latency to first bleat, success to stand and suck, were registered in the lambs. PPA ewes registered shorter duration of labour (19.2 ± 4.2 vs. 42.6 ± 7.8 min), earlier onset of grooming (1.2 ± 0.4 vs. 3.0 ± 0.6 min), higher MBS (4.5 ± 0.1 vs. 3.1 ± 0.4) and did not desert any lamb during the first 72 h from birth. The lambs born to PPA mothers registered higher O2 saturation (97.6 ± 1.0 % vs. 93.4 ± 1.3 %), bleated earlier (2.4 ± 0.5 vs. 4.6 ± 0.8 min), stood up earlier (24.1 ± 4.2 vs. 36.8 ± ... Presentar Todo |
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Palabras claves : |
Birth asphyxia; Delivery assistance; Ewe; Lamb; Maternal behaviour. |
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Asunto categoría : |
L10 Genética y mejoramiento animal |
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Marc : |
LEADER 03234naa a2200277 a 4500
001 1064215
005 2023-06-30
008 2023 bl uuuu u00u1 u #d
022 $a0168-1591 (print); 1872-9045 (electronic).
024 7 $a10.1016/j.applanim.2023.105980$2DOI
100 1 $aREGUEIRO, M.
245 $aProgrammed parturition assistance (PPA) in primiparous wool-type ewes improves mother-lamb behaviour at lambing.$h[electronic resource]
260 $c2023
500 $aArticle history: Received 6 January 2023; Received in revised form 6 May 2023; Accepted 4 June 2023; Available online 7 June 2023. -- Corresponding author: E-mail address: mariel@fagro.edu.uy (M. Regueiro). --
520 $aThe effect of programmed assistance at parturition on the behaviour of the mother and its offspring in primiparous ewes under extensive rearing was evaluated. Programmed parturition assistance was defined as the manoeuvres performed immediately after the beginning of the expulsion phase to shorten it. Twenty-eight 2-year-old Corriedale primiparous ewes (body condition score (BCS): 3.4 ± 0.1; body weight (BW): 35.5 ± 0.7 kg) with a single foetus were used. Before lambing and considering BW, BCS and sire, the ewes were randomly assigned to (i) Programmed-parturition-assisted (PPA; n = 14): ewes whose lambing was programmed to be assisted or (ii) Not-assisted (NA; n = 14): ewes that were not assisted and their lambs were born through natural labour. The duration of the foetus expulsion phase, maternal behaviour score (MBS, 1-5), onset of grooming, lamb/ewe BW ratio and lamb desertion were determined in the ewes. Birth weight, Apgar test (score 0-10), O2 saturation, meconium-stained coat, latency to first bleat, success to stand and suck, were registered in the lambs. PPA ewes registered shorter duration of labour (19.2 ± 4.2 vs. 42.6 ± 7.8 min), earlier onset of grooming (1.2 ± 0.4 vs. 3.0 ± 0.6 min), higher MBS (4.5 ± 0.1 vs. 3.1 ± 0.4) and did not desert any lamb during the first 72 h from birth. The lambs born to PPA mothers registered higher O2 saturation (97.6 ± 1.0 % vs. 93.4 ± 1.3 %), bleated earlier (2.4 ± 0.5 vs. 4.6 ± 0.8 min), stood up earlier (24.1 ± 4.2 vs. 36.8 ± 8.0 min) and recorded shorter time to suck (36.5 ± 6.7 vs. 71.0 ± 12.9 min). No effect of treatment on Apgar test or meconium-stained coat was observed, but regardless of treatment, meconium-stained lambs had a higher lamb/ewe BW ratio than unstained ones. The reduction of the duration of foetal expulsion phase, through programmed parturition assistance, positively affected the vigour of the lambs as well as the maternal behaviour of primiparous ewes, which in turn would increase the chances of lamb survival and ultimately, improve the welfare of the ewe-lamb unit. © 2023 Elsevier B.V. All rights reserved.
653 $aBirth asphyxia
653 $aDelivery assistance
653 $aEwe
653 $aLamb
653 $aMaternal behaviour
700 1 $aJORGE-SMEDING, E.
700 1 $aBALDI, F.
700 1 $aIDIARTE BORDA, A.
700 1 $aLÓPEZ-MAZZ, C.
700 1 $aBANCHERO, G.
773 $tApplied Animal Behaviour Science. August 2023, Vol. 265, 105980. https://doi.org/10.1016/j.applanim.2023.105980
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